Chapter 15 Mitotic Cells as a Source of Synchronized Cultures1

摘要: Publisher Summary Mitotic cells are of great interest as members the most distinctive phase cellular life cycle and such, have been intensively studied. Of numerous methods available for inducing synchrony in mammalian cells, elegant its simplicity is Tolmach method selective detachment mitotic from a monolayer. Tightly phased populations reasonable size can be produced at beginning with virtually no delay apparent biochemical perturbation. The selection process follows predictions simple model, quality separation continuously monitored, adjustments several variable parameters made on basis volume spectrometry using modification Coulter counter described detail. This chapter discusses different view— namely, preparation utilization these source synchronized cultures which to investigate biochemistry “interphase.” spectacular events mitosis occur only after extensive preparations during interphase, not least important replication genome DNA-synthetic period S.