Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells.
作者： R. A. Tobey , E. C. Anderson , D. F. Petersen
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摘要: A technique has been developed for the selective detachment of mitotic cells from monolayer cultures Chiness hamster with a simple reciprocating shaking machine. Cultures prepared by shake treatment and placed in spinner flasks are routinely obtained, which fraction drops 0.95–0.05 19 minutes. Some properties this described, along procedure producing large quantities cells. Cells chilled immediately after collection series treatments complete mitosis synchronously upon subsequent resuspension warm medium.
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