Chapter 7: Isolation of Mutants of Cultured Mammalian Cells
作者： Larry H. Thompson , Raymond M. Baker
DOI: 10.1016/S0091-679X(08)60052-7
关键词: Gene 、 Ploidy 、 Genotype 、 Mutant 、 Biology 、 Isolation (microbiology) 、 Genetics 、 Molecular genetics 、 Phenotype 、 Eukaryotic cell
摘要: Publisher Summary This chapter discusses the isolation of mutants cultured mammalian cells. The progress molecular genetics has been firmly rooted in technology for isolating mutations affecting diverse biological functions and manipulating mutant genes order to study consequences changes. Isolation cells combined with development new genetic techniques should be invaluable building an understanding fundamental aspects eukaryotic cell growth differentiation. probable redundancy information diploid origin implies that observation phenotypes will infrequent compared occurrence genotypes. characterization altered protein as evidence a gene lacking lines induced culture.
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