Restoring the biophysical properties of decellularized patches through recellularization

摘要: Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for ‘Functional tissue engineering’ (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure composition of ECM—previously shown to affect cell survival, proliferation, migration, organization, differentiation maturation. Inversely, effects cells on ECM constructs’ biophysical properties, under physiological-like conditions, remain still largely unknown. We hypothesized that by re-cellularizing porcine cardiac (pcECM, model scaffold) some original properties myocardial can be restored, which are related scaffold's surface bulk modifications consequent cellularization. performed systematic assessment pcECM scaffolds seeded with human mesenchymal stem (MSCs), common multipotent source in regenerative medicine. report new type FTE study interactions composite-scaffold evaluated from perspective their contribution construct (FTIR, WETSEM™) (DSC, TGA, mechanical testing). results obtained compared acellular native ventricular serving negative positive controls, respectively. MSC recellularization resulted an inter-fiber plasticization effect, increased protein density, masking acylated glycosaminoglycans (GAGs) active remodelling further stabilized reseeded its denaturation resistance. approach presented herein, therefore, identifies “biological plasticizers” yields important methodologies, understanding, data both reference well possible ‘design criteria’ future studies FTE.