Decellularized ECM effects on human mesenchymal stem cell stemness and differentiation.

摘要: Abstract Microenvironment extracellular matrices (ECMs) influence cell adhesion, proliferation and differentiation. The ECMs of different microenvironments have distinctive compositions architectures. This investigation addresses effects deposited by a variety types decellularized with cold-EDTA protocol on multipotent human mesenchymal stromal/stem (hMSC) behavior removes intact cells from ECM, minimal ECM damage contamination. cultured hMSCs, osteogenic two smooth muscle (SMC) lines were tested for the differentiation early passage (‘naive’) hMSC plated ECMs. Uninduced enhanced naive motility while maintaining stemness. Decellularized hMSCs in process stimulated osteogenesis substrate biomineralization absence added dexamethasone, but this induction potential was lower later process. induced to become cell-like phenotypic characteristics contractile synthetic cells. demonstrates useful approach obtaining functional cell-deposited highlights importance specificity influencing stem behavior.