Functional co-expression of CYP2D6 and human NADPH-cytochrome P450 reductase in Escherichia coli.

作者: Michael P. Pritchard , Michael J. Glancey , Jennifer A.R. Blake , David E. Gilham , Brian Burchell

DOI: 10.1097/00008571-199802000-00005

关键词: BiochemistryBiologyMonooxygenaseEscherichia coliDrug metabolismCytochrome P450MicrosomeBufuralolMolecular biologyEnzymeHeterologous expression

摘要: … been co-expressed with human NADPH-cytochrome P450 … to couple to form a NADPH-dependent monooxygenase which … coli cells, even in the absence of exogenous NADPH, were …

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