Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
作者： Naoki Takahashi , Yasuhide Yamada , Hirokazu Taniguchi , Masaru Fukahori , Yusuke Sasaki
关键词: Mutation 、 Poor prognosis 、 Oncology 、 KRAS 、 Clinical significance 、 Internal medicine 、 Clinicopathological features 、 Gene 、 Pathology 、 Medicine 、 Advanced gastric cancer 、 Neuroblastoma RAS viral oncogene homolog
摘要: RAS-RAF-MEK-ERK and PI3K-AKT pathways form a significant cascade for potential molecular target therapy in advanced cancer. The clinical significance of mutations these genes gastric cancer (AGC) is uncertain. We collected formalin-fixed, paraffin-embedded fresh frozen tumor samples from AGC patients analyzed the KRAS, NRAS, BRAF PIK3CA by direct-sequencing. retrospectively investigated clinicopathological features patients, selected with metastatic Among 167 KRAS codons 12/13 (N = 8/164, 4.9%), (N = 9/163, 5.5%), NRAS codon 12/13(N = 3/159, 1.9%) were detected. Comparison mutated PIK3CA, an all-wild type showed that frequency intestinal was significantly higher whose tissue contained (P = 0.014). 125 cancer, their tumors had shorter overall survival compared wild-type (MST: 14.7 vs 8.8 months, P = 0.011). By multivariate analyses, mutation indicator poor prognosis (adjusted HR 5.607, 95% CI: 1.637-19.203). Our study indicated rare AGC. likely to associate state but further validation other research required.
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