Fungal community analysis in the deep-sea sediments of the central Indian Basin by culture-independent approach

作者: Purnima Singh , Chandralata Raghukumar , Pankaj Verma , Yogesh Shouche

DOI: 10.1007/S00248-010-9765-8

关键词: Hydrostatic pressureTaxonBiologyBiodiversityEcologyAscomycotaMicrobial ecologyBotanyPhylotypeBasidiomycotaMarine fungi

摘要: Few studies have addressed the occurrence of fungi in deep-sea sediments, characterized by elevated hydrostatic pressure, low temperature, and fluctuating nutrient conditions. We evaluated diversity at three locations Central Indian Basin (CIB) a depth ~5,000 m using culture-independent approach. Community DNA isolated from these sediments was amplified universal fungal-specific internal transcribed spacers 18S rDNA primer pairs. A total 39 fungal operational taxonomic units, with 32 distinct taxa were recovered 768 clones generated 16 environmental clone libraries. The application multiple primers enabled recovery eight sequences that appeared to be new. majority belonged diverse phylotypes Ascomycota Basidiomycota. Our results suggested existence cosmopolitan marine CIB. This study further demonstrated varied spatially Individual set amplify different occasionally. is first report on Ocean.

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