Diagnosis of Canine Lymphoid Neoplasia Using Clonal Rearrangements of Antigen Receptor Genes

摘要: Although the diagnosis of canine leukemia and lymphoma in advanced stages is usually uncomplicated, some presentations disease can be a diagnostic challenge. In certain situations, difficult to distinguish from benign reactive proliferation lymphocytes. Because clonality hallmark malignancy, we have developed an assay that uses polymerase chain reaction amplify variable regions immunoglobulin genes T-cell receptor detect presence clonal lymphocyte population. The detected clonally rearranged antigen 91% 77 dogs with lymphoid malignancy. Of 24 tested, were either healthy or had clearly defined conditions not related gene was found one (a dog Ehrlichia canis infection). Gene rearrangement appropriate for immunophenotype (immunoglobulin B-cell leukemias leukemias). Dilution analysis showed could when 0.1-10% DNA derived neoplastic cells, depending on source tissue. Potential applications this include biopsy samples, cavity fluids, fine needle aspirates, bone marrow peripheral blood; determination lineage (B T cell); staging lymphoma; detection residual after chemotherapy.