Direct Visualization of Antigen-specific CD8+T Cells during the Primary Immune Response to Epstein-Barr Virus In Vivo
作者： M.F.C. Callan , L. Tan , N. Annels , G.S. Ogg , J.D.K. Wilson
关键词:
摘要: Primary infection with virus can stimulate a vigorous cytotoxic T cell response. The magnitude of the antigen-specific component versus bystander primary response remains controversial. In this study, we have used tetrameric major histocompatibility complex–peptide complexes to directly visualize cluster differentration (CD)8+ cells during immune Epstein-Barr (EBV) in humans. We show that massive expansion activated, occurs virus. one individual, specific for single EBV epitope comprised 44% total CD8+ within peripheral blood. majority had an activated/memory phenotype, expression human leukocyte antigen (HLA) DR, CD38, and CD45RO, downregulation CD62 (CD62L), low levels CD45RA. After recovery from AIM, frequency fell most donors studied, although populations continued be easily detectable at least 3 yr.
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