Single-cell PCR analysis of TCR repertoires selected by antigen in vivo: a high magnitude CD8 response is comprised of very few clones.
作者： Janet L Maryanski , C Victor Jongeneel , Philipp Bucher , Jean-Laurent Casanova , Paul R Walker
DOI: 10.1016/S1074-7613(00)80297-6
关键词:
摘要: Taking advantage of a potent MHC class I-restricted response that allows the identification antigen-selected CD8 T cells directly ex vivo, we characterized antigen-specific cell repertoires develop in individual mice by single-cell PCR analysis. Each immune displayed distinct yet structurally similar TCR repertoires. The overall repertoire size was estimated to be range 15-20 for most mice. No major differences were observed between primary and secondary responses. Moreover, hyperimmunized mouse expressed 8 months after initial immunization very found at peak response. Our results demonstrate high magnitude may composed few clones, least system analyzed, memory largely reflects selected
elsevier.com
sci-hub.se 参考文章(34)
Peter K. Gregersen, Robert Pergolizzi, In-Hong Choi, Ravi Hingorani, Beena Gulwani-Akolkar, Pradip Akolkar, Jack Silver, Clonal predominance of T cell receptors within the CD8+ CD45RO+ subset in normal human subjects. Journal of Immunology. ,vol. 151, pp. 5762- 5769 ,(1993)
R. Küppers, M. Zhao, M.L. Hansmann, K. Rajewsky, Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections. The EMBO Journal. ,vol. 12, pp. 4955- 4967 ,(1993) , 10.1002/J.1460-2075.1993.TB06189.X
C. Chothia, D. R. Boswell, A. M. Lesk, The outline structure of the T-cell alpha beta receptor. The EMBO Journal. ,vol. 7, pp. 3745- 3755 ,(1988) , 10.1002/J.1460-2075.1988.TB03258.X
Bertrand Jordan, Rs. Accolla, Jl. Maryanski, H2-restricted Recognition of Cloned Hla Class-i Gene-products Expressed in Mouse Cells Journal of Immunology. ,vol. 136, pp. 4340- 4347 ,(1986)
Sarah Rowland-Jones, John I. Bell, Andrew J. McMichael, Margaret F. C. Callan, Hugh T. Reyburn, Paul Bowness, Selection of T cell receptor variable gene-encoded amino acids on the third binding site loop: a factor influencing variable chain selection in a T cell response. European Journal of Immunology. ,vol. 25, pp. 1529- 1534 ,(1995) , 10.1002/EJI.1830250609
Biao Zheng, Wei Xue, Garnett Kelsoe, Locus-specific somatic hypermutation in germinal centre T cells. Nature. ,vol. 372, pp. 556- 559 ,(1994) , 10.1038/372556A0
Nicholas R. J. Gascoigne, Yueh-hsiu Chien, Daniel M. Becker, Joshua Kavaler, Mark M. Davis, Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genes Nature. ,vol. 310, pp. 387- 391 ,(1984) , 10.1038/310387A0
Gerald Siu, Mitchell Kronenberg, Erich Strauss, Regina Haars, Tak W. Mak, Leroy Hood, The structure, rearrangement and expression of D beta gene segments of the murine T-cell antigen receptor. Nature. ,vol. 311, pp. 344- 349 ,(1984) , 10.1038/311344A0
JL Casanova, F Martinon, H Gournier, C Barra, C Pannetier, A Regnault, P Kourilsky, JC Cerottini, JL Maryanski, None, T cell receptor selection by and recognition of two class I major histocompatibility complex-restricted antigenic peptides that differ at a single position. Journal of Experimental Medicine. ,vol. 177, pp. 811- 820 ,(1993) , 10.1084/JEM.177.3.811
M. Behlke, D. Spinella, H. Chou, W Sha, D. Hartl, D. Loh, T-cell receptor beta-chain expression: dependence on relatively few variable region genes Science. ,vol. 229, pp. 566- 570 ,(1985) , 10.1126/SCIENCE.3875151