H2-restricted Recognition of Cloned Hla Class-i Gene-products Expressed in Mouse Cells

摘要: Long-term syngeneic mouse cytolytic T lymphocyte (CTL) clones were obtained from DBA/2 (H2d) mice immunized with P815 cells transfected cloned human class I histocompatibility genes, HLA-CW3 or HLA-A24. Three distinct patterns of specificity defined on HLA transfectant target cells. One clone lysed but not -A24 transfectants, and a second HLA-A24 -CW3 The third targets either gene. None the CTL L (H2k) same genes that expressed these specificities. Several lines evidence indicated recognition transfectants by was H2 restricted. First, lysis could be inhibited anti-H2Kd monoclonal antibody. In addition, anti-P815-HLA lyse (human X mouse) hybrid both H2Kd antigens, clonal derivative no longer H2Kd. most direct for H2-restricted P815-HLA double transfection genes. susceptible to corresponding targets. Thus under certain conditions, xenogeneic gene products can restricted other products.