Complete foldback transposable elements encode a novel protein found in Drosophila melanogaster.

摘要: An apparently complete foldback (FB) transposable element homologous to FB white-crimson (FBwc) was analyzed. A could encode one or more proteins required for regulation of transposition. The central DNA region (the loop) and the junctions between loop inverted terminal repeats were sequenced. Three open reading frames (ORFs) are present in loop, a novel 308 bp repeat is at junctions. No significant homologies found when sequences screened against Gene data bank. Antibodies prepared guinea-pigs peptide near amino terminus ORF1, longest ORF. 71,000 dalton protein isolated from an extract Drosophila melanogaster early-stage embryos on anti-ORF1 peptide-affinity column. Immunohistochemical studies adult flies demonstrate localization this egg chambers.