Optical Imaging: How Far Can We Go

摘要: Optical imaging comprises a broad and diverse range of technology modalities, which aim to assess the morphology, dynamics, pathophysiology state organs, tissues, cells, organelles, molecules, in their natural real time. The development pioneer instruments for optical is referenced seventeenth- eighteenth-century era (circa 1632–1723), when it presumed that Antonie van Leeuwenhoek invented used prototype light microscope (Wollman AJM, Nudd R, Hedlund EG, Leake MC, Open Biol 5:150019, http://dx.doi.org/10.1098/rsob.150019, 2016). groundbreaking discovery its application was reported by Robert Hooke (Hooke Micrographia: or some physiological descriptions minute bodies made magnifying glasses, with observations inquiries thereupon. Courier Corporation, New York, 1665; Baker H, M, Philos Trans 41:503–519, doi: 10.1098/rstl.1739.0085, 1739), who combination stage, source, lenses similar those currently Fast-forward twentieth twenty-first century, innovative advances underscore extraordinary progression into technologies, such as 3-D electron microscopy, confocal fluorescent imaging, mass spectrometry, bioluminescence, optoacoustics, name few (Weissleder Nahrendorf M. Proc Natl Acad Sci 112(47):14424–14428, 2015). Collectively, new modalities enable researchers reveal complex structures dynamic interactive processes happening deep inside cellular compartments, can provide invaluable basic clinical science information. present chapter outlines over three centuries technology, relates rationale led methods, have transformed means observe, analyze, study, diagnose nature functions. paradigm shift inherent progressive impact on bench bedside applications are accordingly discussed.