Mass Cytometry to Decipher the Mechanism of Nongenetic Drug Resistance in Cancer

摘要: Nongenetic resistance has recently been described as a major impediment to effective cancer therapy. is challenging study since it occurs nonuniformly, even in cell lines, and can involve the interplay of multiple survival pathways. Until recently, no technology allowed measurement large-scale alterations pathways with single-cell resolution. Mass cytometry, flow-based technique which activation up 50 proteins be measured simultaneously single-cell, now provides ability examine nongenetic on functional level cell-by-cell basis. The application mass combination new bioinformatic techniques, will allow fundamental questions addressed: Is caused by selection cells pre-existing phenotype or induction program? Which are necessary for how do they interact? Currently, cytometry being used investigate mechanism TRAIL-induced apoptosis. approaches developed understand TRAIL likely applied elucidate mechanisms broadly clinic.