Single-cell phospho-protein analysis by flow cytometry.

摘要: This protocol describes methods for monitoring intracellular phosphorylation-dependent signaling events on a single-cell basis. approach measures cell by treating cells with exogenous stimuli, fixing formaldehyde, permeabilizing methanol, and then staining phospho-specific antibodies. Thus, states can be determined as measure of how interact their environment. method has applications in clinical research well mechanistic studies basic biology. In research, diagnostic or drug efficacy information retrieved discovering disease affects the ability to respond growth factors. Basic scientists use this technique analyze lines human murine primary cells, including rare populations, like B1 stem cells. broad bringing standard biochemical analysis into order garner valuable about physiologic settings.