Coordinate Analysis of Murine Immune Cell Surface Markers and Intracellular Phosphoproteins by Flow Cytometry
作者： Peter O. Krutzik , Matthew R. Clutter , Garry P. Nolan
DOI: 10.4049/JIMMUNOL.175.4.2357
关键词: Cell type 、 Intracellular 、 Monocyte 、 Phosphoprotein 、 Molecular biology 、 Cluster of differentiation 、 Cell 、 Staining 、 Biology 、 Flow cytometry
摘要: … To highlight the utility of phosphospecific flow cytometry in analyzing immune cell signaling, we applied the technique to analyze IL-2, IL-6, IL-10, and IL-15 signaling in murine …
aai.org
jimmunol.org 参考文章(20)
Omar D. Perez, Peter O. Krutzik, Garry P. Nolan, Flow Cytometric Analysis of Kinase Signaling Cascades Methods of Molecular Biology. ,vol. 263, pp. 67- 94 ,(2004) , 10.1385/1-59259-773-4:067
Van De Wiele, C Justin, Julie H Marino, Bryce W Murray, Stephen S Vo, Michael E Whetsell, T Kent Teague, Thymocytes between the β-Selection and Positive Selection Checkpoints Are Nonresponsive to IL-7 as Assessed by STAT-5 Phosphorylation Journal of Immunology. ,vol. 172, pp. 4235- 4244 ,(2004) , 10.4049/JIMMUNOL.172.7.4235
Omar D. Perez, Garry P. Nolan, Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry Nature Biotechnology. ,vol. 20, pp. 155- 162 ,(2002) , 10.1038/NBT0202-155
Ellen N Kersh, Susan M Kaech, Thandi M Onami, Miriana Moran, E John Wherry, M Carrie Miceli, Rafi Ahmed, None, TCR Signal Transduction in Antigen-Specific Memory CD8 T Cells Journal of Immunology. ,vol. 170, pp. 5455- 5463 ,(2003) , 10.4049/JIMMUNOL.170.11.5455
Steven K. Koester, Wade E. Bolton, Chapter 12 Strategies for cell permeabilization and fixation in detecting surface and intracellular antigens Cytometry. ,vol. 63, pp. 253- 268 ,(2001) , 10.1016/S0091-679X(01)63016-4
James W. Jacobberger, R. Michael Sramkoski, Phyllis S. Frisa, Peggy Peng Ye, Megan A. Gottlieb, David W. Hedley, T. Vincent Shankey, Bradley L. Smith, Mary Paniagua, Charles L. Goolsby, Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity. Cytometry Part A. ,vol. 54, pp. 75- 88 ,(2003) , 10.1002/CYTO.A.10063
Jonathan M Irish, Randi Hovland, Peter O Krutzik, Omar D Perez, Øystein Bruserud, Bjørn T Gjertsen, Garry P Nolan, Single Cell Profiling of Potentiated Phospho-Protein Networks in Cancer Cells Cell. ,vol. 118, pp. 217- 228 ,(2004) , 10.1016/J.CELL.2004.06.028
Gülbû Uzel, David M. Frucht, Thomas A. Fleisher, Steven M. Holland, Detection of Intracellular Phosphorylated STAT-4 by Flow Cytometry Clinical Immunology. ,vol. 100, pp. 270- 276 ,(2001) , 10.1006/CLIM.2001.5078
Pietro Pala, Tracy Hussell, Peter J.M. Openshaw, Flow cytometric measurement of intracellular cytokines. Journal of Immunological Methods. ,vol. 243, pp. 107- 124 ,(2000) , 10.1016/S0022-1759(00)00230-1
Agnese A. Pollice, J. Philip Mccoy, Stanley E. Shackney, Charles A. Smith, Jyotsna Agarwal, Dennis R. Burnolt, Laura E. Janocko, Francis J. Hornicek, Sarita G. Singh, Robert J. Hartsock, Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins. Cytometry. ,vol. 13, pp. 432- 444 ,(1992) , 10.1002/CYTO.990130414