Detection of Intracellular Phosphorylated STAT-4 by Flow Cytometry

摘要: The convergence of innate and adaptive immunity is critical for host defense, allowing early protection the generation specific responses. STAT-4 at that point convergence, unifying IFNalpha IL-12 pathways. Activation crucial to T cell polarization, B NK activation, control intracellular pathogens. However, techniques detect phosphorylated are cumbersome require many cells. We have developed a flow cytometric detection technique investigate signaling in human peripheral blood mononuclear Using different polyclonal antibodies recognize either total protein or tyrosine-phosphorylated STAT-4, we can easily PHA/IL-2 blasts derived from lymphocytes. This not only allows us evaluate signaling, but it also less time consuming labor intensive than alternative methods. this cytometry-based method, should be able patients with defects receptor signal transduction, who typically present disseminated nontuberculous mycobacterial infections.