摘要: Huhnereiweis-Lysozym war das erste Enzym, dessen Tertiarstruktur aufgeklart werden konnte. Seine Peptidkette ist in zwei etwa gleich grosen Teilen angeordnet, zwischen denen ein tiefer Spalt klafft. Die Substrate (und Inhibitoren) diesem durch Wasserstoffbrucken gebunden und unter Mitwirkung von Glu 35 Asp 52 — die aktive Zentrum bilden hydrolysiert. Lysozyme, vielen Tier- Pflanzenarten vorkommen, sind zwar chemisch verschieden, haben jedoch qualitativ gleiche biologische Aktivitat; quantitativ lassen sich grose Unterschiede nachweisen, auch Spezifitat betreffen. Nach Infektion mit Bakteriophagen entsteht E. coli Lysozym, Bildung der Phagen-DNS kontrolliert wird. Da mutierte Phagen abgeanderte Lysozyme erzeugen, eroffnet weites Feld fur molekularbiologische Forschung.
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