Eukaryotic transporters for hydroxyderivatives of benzoic acid

摘要: Several yeast species catabolize hydroxyderivatives of benzoic acid. However, the nature carriers responsible for transport these compounds across plasma membrane is currently unknown. In this study, we analyzed a family genes coding permeases belonging to major facilitator superfamily (MFS) in pathogenic Candida parapsilosis. Our results revealed that transporters are functionally equivalent bacterial aromatic acid: H+ symporters (AAHS) such as GenK, MhbT and PcaK. We demonstrate HBT1 HBT2 encoding putative highly upregulated C. parapsilosis cells assimilating hydroxybenzoate substrates corresponding proteins reside membrane. Phenotypic analyses knockout mutants uptake assays provide compelling evidence Hbt1 Hbt2 metabolized via gentisate (3-hydroxybenzoate, gentisate) 3-oxoadipate pathway (4-hydroxybenzoate, 2,4-dihydroxybenzoate protocatechuate), respectively. data support hypothesis belong AAHS MFS transporters. Phylogenetic orthologs Hbt widespread subphylum Pezizomycotina, but have sparse distribution among Saccharomycotina lineages. Moreover, shed additional light on evolution biochemical pathways involved catabolic degradation hydroxyaromatic compounds.