Devitalisation of human cartilage by high hydrostatic pressure treatment: Subsequent cultivation of chondrocytes and mesenchymal stem cells on the devitalised tissue.

摘要: The regeneration of cartilage lesions still represents a major challenge. Cartilage has tissue-specific architecture, complicating recreation by synthetic biomaterials. A novel approach for reconstruction is the use devitalised cartilage. Treatment with high hydrostatic pressure (HHP) achieves devitalisation while biomechanical properties are remained. Therefore, in present study, was using HHP treatment and potential revitalisation chondrocytes mesenchymal stem cells (MSCs) investigated. performed application 480 MPa over 10 minutes. Effective cellular inactivation demonstrated trypan blue exclusion test DNA quantification. Histology electron microscopy examinations showed undamaged structure after treatment. For MSCs were cultured on without supplementation chondrogenic growth factors. Both significantly increased expression cartilage-specific genes. ECM stainings neocartilage-like positive AZAN staining as well collagen type II aggrecan deposition three weeks cultivation. Our results that caused tissue. proteins not influenced, thus, providing scaffold differentiation chondrocytes. HHP-treated tissue might be promising repair.