Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities.
作者： Mayu Inokuchi , Junya Hiroi , Soichi Watanabe , Kyung Mi Lee , Toyoji Kaneko
DOI: 10.1016/J.CBPA.2008.06.012
关键词: Endocrinology 、 Tilapia 、 Biology 、 Oreochromis mossambicus 、 Population 、 Molecular biology 、 Apical membrane 、 Internal medicine 、 Gill 、 Cotransporter 、 Na+/K+-ATPase 、 Salinity
摘要: Abstract We explored molecular and morphological alteration in gill mitochondria-rich (MR) cells of Mozambique tilapia, Oreochromis mossambicus , acclimated to deionized freshwater (DFW), (FW), 1/3-diluted seawater (1/3 SW) (SW). Scanning electron microscopic observations revealed that the apical membrane MR appeared as a flat or slightly projecting disk DFW FW, being larger than FW. In contrast, typically formed pit structure 1/3 SW SW. The mRNA expression levels Na + /H exchanger-3 (NHE3) /Cl − cotransporter (NCC) gills were increased with decreasing environmental salinity, whereas /K /2Cl cotransporter-1a (NKCC1a) was upregulated by increasing salinity. Immunofluorescence staining showed cell population DFW- FW-acclimated tilapia consisted mostly NHE3 those apical-NCC; basolateral NKCC1a dominated SW-acclimated tilapia. These results indicated apical-NHE3 apical-NCC ion-absorbing cells, basolateral-NKCC1a ion-secreting cells. fish SW, both secreting existed gills, suggesting near-isotonic water equipped mechanisms hyper- hypoosmoregulation prepare for salinity changes.
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