Stimulation and inhibition of growth by EGF in different A431 cell clones is accompanied by the rapid induction of c-fos and c-myc proto-oncogenes.

摘要: Stimulation of quiescent fibroblasts to growth by polypeptide factors is accompanied the rapid induction c-fos and c-myc proto-oncogenes. In contrast fibroblasts, A431 cells respond epidermal factor (EGF) with a decreased rate. Here we report that, in spite its inhibitory effect, EGF rapidly induces transient expression mRNA, followed synthesis nuclear protein. addition, treatment resulted elevated levels expression. Practically identical results were obtained variant clones that are resistant effect on cell proliferation. These observations suggest primary consequence factor-receptor interaction. Indeed, efficient both genes was also observed cyanide bromide-cleaved EGF, which has previously been shown be non-mitogenic but able trigger early events induced EGF. We strong lesser extent TPA, calcium ionophore A23187, indicating an important role for kinase C proto-oncogene activation factors.