Effects of anisomycin on LTP in the hippocampal CA1: long-term analysis using optical recording.
作者： Hiraku Mochida , Katsushige Sato , Shinichi Sasaki , Itaru Yazawa , Kohtaro Kamino
DOI: 10.1097/00001756-200104170-00025
关键词: Dentate gyrus 、 Internal medicine 、 Hippocampal formation 、 Excitatory postsynaptic potential 、 Anisomycin 、 Chemistry 、 Synaptic plasticity 、 Hippocampus 、 LTP induction 、 Neuroscience 、 Long-term potentiation 、 Endocrinology
摘要: Long-term potentiation (LTP) in the hippocampal CA1 region and dentate gyrus consists of different stages: early LTP lasting minutes or several hours, late longer than 4 h. It has been suggested that phase is dependent on protein synthesis. However, experimental results effects synthesis inhibitors are still confusing. We applied optical recording techniques to rat slices, re-evaluated a inhibitor, anisomycin, LTP. Using voltage-sensitive oxonol dye, NK3630 (RH482), could be monitored optically for long-term period (7-8 h). In presence EPSP (excitatory postsynaptic potential) lasted about 2-3 h, followed by gradual decline signal amplitude. Statistically, significant anisomycin were observed 6 h after induction 100 Hz tetanus 8 400 tetanus. These suggest independent synthesis, while (> 3-5 h) depends
sci-hub.se 参考文章(21)
Peter V. Nguyen, Eric R. Kandel, A Macromolecular Synthesis-Dependent Late Phase of Long-Term Potentiation Requiring cAMP in the Medial Perforant Pathway of Rat Hippocampal Slices The Journal of Neuroscience. ,vol. 16, pp. 3189- 3198 ,(1996) , 10.1523/JNEUROSCI.16-10-03189.1996
Lawrence B. Cohen, Brian M. Salzberg, Optical measurement of membrane potential. Reviews of Physiology Biochemistry and Pharmacology. ,vol. 83, pp. 35- 88 ,(1978) , 10.1007/3-540-08907-1_2
PK Stanton, JM Sarvey, Blockade of long-term potentiation in rat hippocampal CA1 region by inhibitors of protein synthesis The Journal of Neuroscience. ,vol. 4, pp. 3080- 3088 ,(1984) , 10.1523/JNEUROSCI.04-12-03080.1984
P. Nguyen, T Abel, E. Kandel, Requirement of a Critical Period of Transcription for Induction of a Late Phase of LTP Science. ,vol. 265, pp. 1104- 1107 ,(1994) , 10.1126/SCIENCE.8066450
Uwe Frey, Richard G. M. Morris, Synaptic tagging and long-term potentiation Nature. ,vol. 385, pp. 533- 536 ,(1997) , 10.1038/385533A0
Uwe Frey, Manfred Krug, Klaus G. Reymann, Hansjuergen Matthies, Anisomycin, an inhibitor of protein synthesis, blocks late phases of LTP phenomena in the hippocampal CA1 region in vitro. Brain Research. ,vol. 452, pp. 57- 65 ,(1988) , 10.1016/0006-8993(88)90008-X
T TSUMOTO, Long-term potentiation and long-term depression in the neocortex Progress in Neurobiology. ,vol. 39, pp. 209- 228 ,(1992) , 10.1016/0301-0082(92)90011-3
A. Grinvald, A. Manker, M. Segal, Visualization of the spread of electrical activity in rat hippocampal slices by voltage-sensitive optical probes The Journal of Physiology. ,vol. 333, pp. 269- 291 ,(1982) , 10.1113/JPHYSIOL.1982.SP014453
Y Nakagami, H Saito, N Matsuki, Optical recording of trisynaptic pathway in rat hippocampal slices with a voltage-sensitive dye Neuroscience. ,vol. 81, pp. 1- 8 ,(1997) , 10.1016/S0306-4522(97)00161-9
T. V. P. Bliss, G. L. Collingridge, A synaptic model of memory: long-term potentiation in the hippocampus Nature. ,vol. 361, pp. 31- 39 ,(1993) , 10.1038/361031A0