Estimating conductances of dual-recorded neurons within a network of coupled cells.

摘要: Simultaneous pre- and postsynaptic cell recordings are used to calculate gap junction conductance based on an equivalent electrical circuit of electrically coupled pair cells. This calculation is imprecise when recording from a that neighboring cells providing indirect paths between the recorded Despite this imprecision, junctional has been calculated for networks during past 40 years since more accurate method was lacking. The present study simulated three-dimensional network heterogeneous neurons mathematical modeling reduce complexity simplest equations could accurately estimate properties dual-recorded in network. Analyses simulations showed knowledge number unrecorded directly linked voltage responses these were largely sufficient predict direct resistance linking as well input would exist absence coupling. All model parameters be obtained real dual-intracellular penetrations which allow electrophysiological intracellular staining.