Connexin43 is not expressed in principal cells of mouse cortex and hippocampus

摘要: Previous immunofluorescence analyses in mice and rats showed a mainly astrocytic expression of the gap junction protein connexin43 (Cx43) brain. However, situ hybridization murine brain sections suggested strong Cx43 mRNA hippocampal cortical pyramidal neurons Purkinje cells. These findings contrast with recent immunoelectron microscopic studies that excluded prominent neurons. Both contrasting results could be explained by post-transcriptional control mechanisms. Here we demonstrate conditional replacement coding region lacZ reporter gene, mimicking transcriptional activity is not expressed principal cells This histochemical approach used prone to cross-reactivity probes or antibodies. Furthermore, show signals, specific for mouse neurons, are retained even when DNA removed cre-mediated deletion. Our confirm previous adult underscore importance connexin-deficient as controls studies. We found no evidence gene Thus, synchronized neuronal networks cannot depend on containing junctions these