Canine parvovirus NS1 induced apoptosis involves mitochondria, accumulation of reactive oxygen species and activation of caspases.
作者： A.K. Gupta , S.K. , Sahoo , A.P. , Rosh
DOI: 10.1016/J.VIRUSRES.2015.10.019
关键词: Caspase 、 HeLa 、 Programmed cell death 、 Intrinsic apoptosis 、 Biology 、 Cell cycle 、 Apoptosis 、 Cell biology 、 Mitochondrion 、 Reactive oxygen species
摘要: The non-structural protein (NS1) of parvoviruses plays an important role in viral replication and is thought to be responsible for inducing cell death. However, the detailed mechanism pathways involved canine parvovirus type 2 NS1 (CPV2.NS1) induced apoptosis are not yet known. In present study, we report that expression CPV2.NS1 HeLa cells arrests G1 phase cycle mitochondria mediated as indicated by mitochondrial depolarization, release cytochrome-c activation caspase 9. Treatment with 9 inhibitor Z-LEHD-FMK reduced induction significantly. We also causes accumulation reactive oxygen species (ROS) treatment antioxidant reduces ROS levels extent apoptosis. Our results provide insight into apoptosis, which might prove valuable developing oncolytic agent.
elsevier.com
sci-hub.se 参考文章(41)
Stefan Grimm, Mathieu Noteborn, Anticancer genes: inducers of tumour-specific cell death signalling Trends in Molecular Medicine. ,vol. 16, pp. 88- 96 ,(2010) , 10.1016/J.MOLMED.2009.12.002
Anne Op De Beeck, Joelle Sobczak-Thepot, Huseyin Sirma, Florence Bourgain, Christian Brechot, Perrine Caillet-Fauquet, NS1- and Minute Virus of Mice-Induced Cell Cycle Arrest: Involvement of p53 and p21 cip1 Journal of Virology. ,vol. 75, pp. 11071- 11078 ,(2001) , 10.1128/JVI.75.22.11071-11078.2001
N. Sol, J. Le Junter, I. Vassias, J. M. Freyssinier, A. Thomas, A. F. Prigent, B. B. Rudkin, S. Fichelson, F. Morinet, Possible interactions between the NS-1 protein and tumor necrosis factor alpha pathways in erythroid cell apoptosis induced by human parvovirus B19. Journal of Virology. ,vol. 73, pp. 8762- 8770 ,(1999) , 10.1128/JVI.73.10.8762-8770.1999
Jürg P. F. Nüesch, Jean Rommelaere, NS1 Interaction with CKIIα: Novel Protein Complex Mediating Parvovirus-Induced Cytotoxicity Journal of Virology. ,vol. 80, pp. 4729- 4739 ,(2006) , 10.1128/JVI.80.10.4729-4739.2006
Qing Zheng, Chenwen Cai, Di Zhao, Shudong Xiao, Yuanyuan Wang, Specific anti-gastric cancer effects of a recombinant plasmid expressing nonstructural protein 1 of parvovirus H1. Saudi Medical Journal. ,vol. 35, pp. 336- 345 ,(2014)
Jürg P.F. Nüesch, Jeannine Lacroix, Antonio Marchini, Jean Rommelaere, Molecular Pathways: Rodent Parvoviruses—Mechanisms of Oncolysis and Prospects for Clinical Cancer Treatment Clinical Cancer Research. ,vol. 18, pp. 3516- 3523 ,(2012) , 10.1158/1078-0432.CCR-11-2325
Brian D. Poole, Jing Zhou, Amy Grote, Adam Schiffenbauer, Stanley J. Naides, Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein. Journal of Virology. ,vol. 80, pp. 4114- 4121 ,(2006) , 10.1128/JVI.80.8.4114-4121.2006
Eiji Morita, Akitoshi Nakashima, Hironobu Asao, Hiroyuki Sato, Kazuo Sugamura, Human Parvovirus B19 Nonstructural Protein (NS1) Induces Cell Cycle Arrest at G1 Phase Journal of Virology. ,vol. 77, pp. 2915- 2921 ,(2003) , 10.1128/JVI.77.5.2915-2921.2003
Stanley Moffatt, Nobuo Yaegashi, Kohtaro Tada, Nobuyuki Tanaka, Kazuo Sugamura, None, Human Parvovirus B19 Nonstructural (NS1) Protein Induces Apoptosis in Erythroid Lineage Cells Journal of Virology. ,vol. 72, pp. 3018- 3028 ,(1998) , 10.1128/JVI.72.4.3018-3028.1998
Juwar Doley Juwar Doley, LV Singh, GR Kumar, AP Sahoo, Lovleen Saxena Lovleen Saxena, Uttara Chaturvedi Uttara Chaturvedi, Shikha Saxena Shikha Saxena, Rajiv Kumar Rajiv Kumar, PK Singh, RS Rajmani, Lakshman Santra Lakshman Santra, SK Palia, S Tiwari, DR Harish, Arvind Kumar Arvind Kumar, GS Desai, Smita Gupta Smita Gupta, SK Gupta, AK Tiwari, None, Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways Applied Biochemistry and Biotechnology. ,vol. 172, pp. 497- 508 ,(2014) , 10.1007/S12010-013-0538-Y