Combined administration of the apoptin gene and poly (I:C) induces potent anti-tumor immune response and inhibits growth of mouse mammary tumors.

摘要: Abstract Background Many viral proteins exhibit selective cytotoxicity for tumor cells without affecting the normal diploid cells. The apoptin protein of chicken infectious anemia virus is one such proteins, which has been shown to kill specifically. However, an effective cancer treatment strategy also requires assistance from immune system. Recently, poly (I:C) be vaccine adjuvant. Aim In this study, we assessed anti-tumor potential gene transfer alone and in combination with a 4T1 mouse mammary model. Methods were used induce Balb/c mice. Mice bearing tumors divided into 6 groups, each group received six intratumoral injections during period month. After last immunization, animals sacrificed, peripheral blood, spleen, lungs, liver, heart, kidney tissues collected immunological, molecular pathological analysis. Results We report that administration plasmid along not only significantly inhibited growth tumor, but induced potent response as indicated by increase blood CD4 +, CD8 + infiltration tissue. Further, serum analysis cytokines revealed increased secretion Th1 (IFN-γ IL-2). Conclusions results our study demonstrate inclusion enhanced activity mainly inducing response. Therefore, use novel powerful immunotherapy.