Evolution of novel sorbents for effective clean-up of honeybee matrix in highly toxic insecticide LC/MS/MS analysis
作者： Piotr Kaczynski , Izabela Hrynko , Bozena Lozowicka
DOI: 10.1016/J.ECOENV.2017.01.033
关键词: Acetic acid 、 Mass spectrometry 、 Extraction (chemistry) 、 Solvent 、 Analyte 、 Chromatography 、 Matrix (chemical analysis) 、 Chemistry 、 Chitin 、 Quechers
摘要: Abstract Highly toxic insecticides (HTIs) belonging to different chemical groups are dangerous pollinating organisms, even in sublethal doses. An important objective of this study was develop a method determine over fifty HTIs at very low concentrations the bee matrix. The novelty research involved obtaining extract completely free from beewaxes, lipids and proteins using EMR-lipid (enhanced matrix removal-lipid), chitin Z-Sep+ (zirconium oxide C18 dual-bonded silica) as clean-up sorbents. Different parameters, such weight bees, extraction solvent, freezing time were evaluated. Determinations made liquid chromatography-tandem mass spectrometry (LC-MS/MS). allows for removing most fatty co-extracts increases overall performance by reducing effects (ME) without significant analyte loss. established modified QuEChERS based on 1% acetic acid acetonitrile followed validated three spiking levels (0.001, 0.01 0.1 mg kg −1 ). Precision, calculated relative standard deviation (RSD), below 20%. proposed used doses these real samples dead honeybees.
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