Analysis of the mitochondrial D-Loop reveals that neither river boundaries nor geographic distance structure the fine-scale genetic variation of an Amazonian treefrog

摘要: While most anurans have limited vagility and local fidelity, there are some exceptions. In the present study, we used Boana boans, a large treefrog found throughout of Amazon basin, as model organism. We investigated possible isolation B. boans demes located on opposite margins Juruena River their population structure. sampled 14 individuals analyzed mitochondrial D-Loop to verify whether river or Euclidean distance is acting barrier dispersal this frog. The sequencing revealed 12 haplotypes, with global Fst values − 0.079, K2P ranging from − 0.187 0.054, primarily intrapopulation (81.78%) genetic diversity, only 18.22% variation being among populations. Analysis molecular variance Bayesian cluster analysis detected lack structuring within study area. species presented capacity for over long distances in comparison other amphibians, which, together its resistance desiccation reproductive mode, enable disperse across rivers overland. specific case River, many fluvial islands area may also be favorable species.