Somatic embryogenesis for plant production of Abies lasiocarpa

摘要: Seeds of Abies lasiocarpa (Hook.) Nutt. (subalpine fir) were dissected, and the different parts analyzed for elemental composition. The data used to design a novel growth medium initiation somatic embryogenesis. Embryogenic cultures initiated from immature zygotic embryos six open-pollinated families A. on three media. frequency was highest in early mid-July when embryo explants ca. 0.8 mm long. Thereafter response declined rapidly. culture media did not significantly affect frequencies, but subsequent survival dependent medium. On Schenk Hildebrandt medium, many ceased grow died. Several decaying rescued after transfer one new Proliferating could be stimulated produce mature embryos. Of 2510 embryos, 212 (8.4%) converted plants, 35 plants have grown over two ...