Somatic embryogenesis of the hybrid Abies cilicica × Abies cephalonica
作者： J. Korecký , J. Vítámvás
DOI: 10.17221/54/2010-JFS
关键词:
摘要: The interspecific hybrid fir Abies cilicica × cephalonica is based on native species (Loud.) and (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. medium BAP at a concentration 1 mg·l -1 , 1,000 myo-inositol, 500 glutamine, casein was found to be the most suitable initiation for somatic embryogenesis this hybrid. Embryogenic suspensor mass produced from immature mature primary explants. frequency ESM dependent composition medium, plant growth regulators, time seeds collection. im - higher in comparison with zygotic embryos, but results were not significantly different. As maturation evaluated Murashige Skoog which contained 10 abscisic acid, 100 g·l PEG 40 maltose (MM 2 medium).
cabdirect.org
sci-hub.st 参考文章(28)
Thomas Geier, Gerhard Reuther, Astrid Schuller, Somatic embryogenesis from seed explants of Abies alba Plant Cell Tissue and Organ Culture. ,vol. 17, pp. 53- 58 ,(1989) , 10.1007/BF00042281
J. Hrib, B. Vookova, A. Kormutak, Biochemical differences between normal callus and embryogenic suspensor mass of silver fir Biologia Plantarum. ,vol. 39, pp. 507- 513 ,(1997) , 10.1023/A:1000914411896
Sara von Arnold, Izabela Sabala, Peter Bozhkov, Julia Dyachok, Lada Filonova, Developmental pathways of somatic embryogenesis Plant Cell Tissue and Organ Culture. ,vol. 69, pp. 233- 249 ,(2002) , 10.1023/A:1015673200621
B. Vookova, R. Matusova, A. Kormutak, Secondary Somatic Embryogenesis in Abies numidica Biologia plantarum. ,vol. 46, pp. 513- 517 ,(2003) , 10.1023/A:1024899124774
Gregory C. Phillips, O. L. Gamborg, Plant cell, tissue and organ culture - Fundamental methods Springer. ,(1995)
Todd G. Guevin, Edward G. Kirby, Induction of embryogenesis in cultured mature zygotic embryos of Abies fraseri (Pursh) Poir Plant Cell Tissue and Organ Culture. ,vol. 49, pp. 219- 222 ,(1997) , 10.1023/A:1005747026269
Astrid Schuller, Robert Kirchner-Neß, Gerhard Reuther, INTERACTION OF PLANT GROWTH REGULATORS AND ORGANIC C AND N COMPONENTS IN THE FORMATION AND MATURATION OF ABIES ALBA SOMATIC EMBRYOS Plant Cell Tissue and Organ Culture. ,vol. 60, pp. 23- 31 ,(2000) , 10.1023/A:1006429428170
T. Salaj, J. Salaj, Somatic Embryo Formation on Mature Abies alba * Abies cephalonica Zygotic Embryo Explants Biologia plantarum. ,vol. 46, pp. 7- 11 ,(2003) , 10.1023/A:1027312410957
Harald Kvaalen, Ola Gram Daehlen, Anne Tove Rognstad, Borgny Grønstad, Ulrika Egertsdotter, Somatic embryogenesis for plant production of Abies lasiocarpa Canadian Journal of Forest Research. ,vol. 35, pp. 1053- 1060 ,(2005) , 10.1139/X05-035
Jens Viktor Nørgaard, Somatic embryo maturation and plant regeneration in Abies nordmanniana Lk Plant Science. ,vol. 124, pp. 211- 221 ,(1997) , 10.1016/S0168-9452(97)04614-1