作者: T. Salaj , J. Salaj
关键词:
摘要: Somatic embryogenesis was achieved from mature embryos excised stored hybrid Abies alba × cephalonica seeds. Embryogenic tissue formation occurred on SH medium supplemented with 1 mg dm−3 benzyladenine. The of embryogenic influenced by the time storage Initiation frequencies 27.2 – 29.0 % were obtained in isolated 6 month and year Embryos 4-year seeds showed no response. structures appeared surface hypocotyl. They originated without previous callus formation. tissues maintained long-term cultures. After maturation treatment cotyledonary somatic developed germinated small plantlets.