Reactive Chlorinating Species Produced during Neutrophil Activation Target Tissue Plasmalogens PRODUCTION OF THE CHEMOATTRACTANT, 2-CHLOROHEXADECANAL

摘要: Recently α-chloro fatty aldehydes have been shown to be products of reactive chlorinating species targeting the vinyl ether bond plasmalogens utilizing a cell-free system. Accordingly, present experiments were designed show that are produced by activated neutrophils and determine their physiologic effects. A sensitive gas chromatography-mass spectrometry technique was developed detect pentafluorobenzyl oximes negative ion chemical ionization. Phorbol 12-myristate 13-acetate activation resulted in production both 2-chlorohexadecanal 2- chlorooctadecanal through myeloperoxidase-dependent mechanism likely involved 16 18 carbon ether-linked aliphatic groups sn-1 position neutrophil plasmalogens. 2-Chlorohexadecanal also fMLP-treated neutrophils. Additionally, released from targeted endothelial cell resulting production. Physiologically relevant concentrations induced chemotaxisin vitro suggesting may role recruitment. Taken together, these studies demonstrate for first time novel biochemical targets during enhance recruitment areas active inflammation.