Inhibition by reactive aldehydes of superoxide anion radical production from stimulated polymorphonuclear leukocytes and pulmonary alveolar macrophages
作者： Gisela Witz , Nancy J. Lawrie , Marie A. Amoruso , Bernard D. Goldstein
DOI: 10.1016/0006-2952(87)90725-8
关键词:
摘要: Alpha,beta-unsaturated aldehydes such as acrolein (ACR) and crotonaldehyde (CRO) have been shown previously in our laboratory to inhibit the production of superoxide anion radical (O2-) by stimulated phagocytic cells vitro a dose-related manner. Based on known reactivity these compounds towards cellular sulfhydryls (SH), present studies were aimed at investigating SH status relation O2- production. Plasma membrane surface groups measured using carboxypyridinedisulfide monitoring resultant formation mixed disulfides through assay thione released into supernatant fraction. Intracellular non-protein 5,5'-dithiobis-2-nitrobenzoic acid. In both human polymorphonuclear leukocytes (PMN) rat pulmonary alveolar macrophages (PAM) there was decrease soluble after ACR CRO treatment. Propionaldehyde, three-carbon saturated aldehyde, without effect. The greater than SH. addition, PMN PAM preincubated with 5-40 microM ACR, inhibition rate no effect lag time cytochrome c reduction. PMN, addition ACR. These data suggest that changes reactive can modulate activity plasma NADPH oxidase responsible for
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