Leukocyte infiltration and inflammatory antigen expression in cadaveric and living-donor livers before transplant1

摘要: BACKGROUND There is evidence to indicate that organs obtained from cadaveric donors may be injured as a result of inflammatory events occurring at around the time brain death. The aim this study was investigate whether there are differences in expression proinflammatory molecules between and living-donor livers before transplant determine any association with donor factors posttransplant graft function. METHODS A comparison biopsies implantation (n=22) living-related (LRD) (n=10) performed. Cryostat tissue sections were stained antibodies leukocyte subpopulations, adhesion molecules, human antigen class II antigens. RESULTS Significantly higher levels CD3+ lymphocytes (1.5%+/-0.8% vs. 0.5%+/-0.3%; P=0.00004), CD68+ monocytes macrophages (4.0%+/-1.2% 2.7%+/-0.6%; P=0.0003), Fas-ligand staining (4.2%+/-2.6% 1.5%+/-1.1%; P=0.0003) detected compared LRD transplantation. Furthermore, intercellular molecule-1 found associated longer periods ventilation (P=0.01), infection (P=0.013), administration dopamine (P=0.03). Although no neutrophil infiltration livers, significantly (P=0.01). CONCLUSION This demonstrates changes occur during period intensive care. These did not seem affect short-term clinical outcome liver allografts but contribute alloimmune responses impairment function long term.