Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation

作者: E. A. Koulaouzidou , K. . Papazisis , P. Beltes , G. D. Geromicholos , A. H. Kortsarls

DOI: 10.1111/J.1600-9657.1998.TB00834.X

关键词: Cell cultureCytotoxicityRoot canalHemocytometerMouse skinChemistryMolecular biologyPulp (tooth)DentistryCytotoxic T cellIn vitro

摘要: The cytotoxicity of three resin-based root canal sealers (AH26, AH-Plus, Topseal) was evaluated in vitro. experiments included two cell lines, L929 mouse skin fibroblasts and RPC-C2A rat pulp cells. assessed by sulforodamine B (SRB) colorimetric assay hemocytometer viable counting after 24- 48-h exposure. AH26 had a severe cytotoxic effect whilst Topseal AH-Plus showed markedly lower toxic influence on the cells during experimental period.

参考文章(18)
The growth in vitro of single isolated tissue cells. Journal of the National Cancer Institute. ,vol. 9, pp. 229- 246 ,(1948) , 10.1093/JNCI/9.3.229
Katsuhiko Takahara, Atsushi Onodera, Koukichi Matsumoto, Toxicity of root canal sealers on rat bone cells in primary culture. Dental Traumatology. ,vol. 6, pp. 200- 207 ,(1990) , 10.1111/J.1600-9657.1990.TB00419.X
Michael Tagger, Etty Tagger, Periapical reactions to calcium hydroxide-containing sealers and AH 26R in monkeys Dental Traumatology. ,vol. 5, pp. 139- 146 ,(1989) , 10.1111/J.1600-9657.1989.TB00350.X
Larz S.W. Spångberg, Sérgio V. Barbosa, Gary D. Lavigne, AH26 releases formaldehyde Journal of Endodontics. ,vol. 19, pp. 596- 598 ,(1993) , 10.1016/S0099-2399(06)80272-4
M. TAGGER, ETTY TAGGER, Effect of implantaton of AH26 silver‐free in subcutaneous tissue of guinea‐pigs International Endodontic Journal. ,vol. 19, pp. 90- 97 ,(1986) , 10.1111/J.1365-2591.1986.TB00897.X
Kokichi Matsumoto, Koichi Inoue, Akira Matsumoto, The effect of newly developed root canal sealers on rat dental pulp cells in primary culture. Journal of Endodontics. ,vol. 15, pp. 60- 67 ,(1989) , 10.1016/S0099-2399(89)80109-8
Hiroshi Nakamura, Fumio Sakakibara, Yasuaki Matsumoto, Shinei Hirano, Hisaki Hayakawa, Kazuyoshi Sakai, Morris Yip, Study on the cytotoxicity of root canal filling materials Journal of Endodontics. ,vol. 12, pp. 156- 160 ,(1986) , 10.1016/S0099-2399(86)80054-1