Cytotoxicity of two resin-based sealers and a fluoride varnish on human gingival fibroblasts.
作者： Masoud Parirokh , Farshid Reza Forghani , Saeed Asgary , Sara Askarifard , Hamzeh Paseban
关键词: Andrology 、 Fluoride varnish 、 Viability assay 、 Varnish 、 MTT assay 、 Biocompatibility 、 Dentistry 、 Medicine 、 Root canal 、 Serial dilution 、 Cytotoxicity
摘要: Introduction: Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility novel materials. In recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage root canal walls when used as sealer. The aim present study was to compare Duraflur with two popular commonly sealers (AH-Plus AH-26) on human gingival fibroblasts (HGF). Methods Materials: HGFs were incubated different concentrations (1/2, 1/4, 1/8) AH-plus, AH-26, 24 h. percentage cell viability assessed methyl-thiazol-tetrazolium (MTT) assay. data analyzed using one-way ANOVA followed by Student-Newman-Keuls test. level significance set at 0.001. Results: MTT assay showed that higher tested materials resulted in lower HGFs. AH-Plus significantly greater compared AH-26 all dilutions (P 0.001). except 1/2 dilution (P<0.001). Conclusion: Although had better it should still be evaluated further tests such intraosseous subcutaneous implantation.
sbmu.ac.ir参考文章(30)
Masoud Parirokh, Mohammad Talebizad, Farshid Reza Forghani, Ali Akabar Haghdoost, Saeed Asgary, Mohammad Jafar Eghbal, Jamileh Goddousi, None, Fluoride Varnish as Root Canal Sealer: A Scanning Electron Microscopy and Bacterial Penetration Study iranian endodontic journal. ,vol. 10, pp. 64- 68 ,(2015) , 10.22037/IEJ.V10I1.5900
Sahar Shakouie, Saeed Rahimi, Hadi Mokhtari, Amin Salem Milani, Negin Ghasemi, Mohammad Forough Reyhani, Hossein Safarvand, Push-Out Bond Strength of Dorifill, Epiphany and MTA-Fillapex Sealers to Root Canal Dentin with and without Smear Layer. iranian endodontic journal. ,vol. 9, pp. 246- 250 ,(2014) , 10.22037/IEJ.V9I4.5655
Mohammad A. Mozayeni, Amin S. Milani, Laleh A. Marvasti, Saeed Asgary, Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material. Australian Endodontic Journal. ,vol. 38, pp. 70- 75 ,(2012) , 10.1111/J.1747-4477.2010.00269.X
P. Yan, Z. Yuan, H. Jiang, B. Peng, Z. Bian, Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. International Endodontic Journal. ,vol. 43, pp. 1116- 1121 ,(2010) , 10.1111/J.1365-2591.2010.01786.X
Fahimeh Sadat Tabatabaei, Seddigheh Naderi, Zahra Jaberiansari, Cytotoxic Effects of Various Mineral Trioxide Aggregate Formulations, Calcium-Enriched Mixture and a New Cement on Human Pulp Stem Cells iranian endodontic journal. ,vol. 9, pp. 271- 276 ,(2014) , 10.22037/IEJ.V9I4.5995
João Carlos Gabrielli Biffi, Paula Dechichi, Virgínia Oliveira Crema, Thais Cristina Cunha, Camilla Christian Gomes Moura, A Study on Biocompatibility of Three Endodontic Sealers: Intensity and Duration of Tissue Irritation iranian endodontic journal. ,vol. 9, pp. 137- 143 ,(2014) , 10.22037/IEJ.V9I2.4962
Setareh Derakhshan, Alireza Adl, Masoud Parirokh, Fatemeh MashadiAbbas, Ali Akbar Haghdoost, None, Comparing subcutaneous tissue responses to freshly mixed and set root canal sealers. iranian endodontic journal. ,vol. 4, pp. 152- 157 ,(2009) , 10.22037/IEJ.V4I4.1492
Esra Pamukcu Guven, Mehmet Emir Yalvac, Mehmet Baybora Kayahan, Hakk Sunay, Fikrettin SahIn, Gunduz Bayirli, Human tooth germ stem cell response to calcium-silicate based endodontic cements Journal of Applied Oral Science. ,vol. 21, pp. 351- 357 ,(2013) , 10.1590/1678-775720130047
E. A. Koulaouzidou, K. . Papazisis, P. Beltes, G. D. Geromicholos, A. H. Kortsarls, Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation Endodontics & Dental Traumatology. ,vol. 14, pp. 182- 185 ,(2007) , 10.1111/J.1600-9657.1998.TB00834.X
F.-M. Huang, K.-W. Tai, M.-Y. Chou, Y.-C. Chang, Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. International Endodontic Journal. ,vol. 35, pp. 153- 158 ,(2002) , 10.1046/J.1365-2591.2002.00459.X