Visualization of Cellulose Synthase Demonstrates Functional Association with Microtubules
作者： Alexander R Paredez , Christopher R Somerville , David W Ehrhardt
关键词: Biophysics 、 Cytoskeleton 、 Microtubule polymerization 、 Biology 、 Biochemistry 、 Cellulose synthase complex 、 Yellow fluorescent protein 、 Microtubule 、 Tubulin 、 Cellulose 、 Cortical microtubule
摘要: Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic Arabidopsis plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and …
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