Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin.
作者： Mohsen Tafaghodi , Bahman Khameneh , Maryam Mohajer
关键词: PLGA 、 Saponin 、 Antigen 、 Particle size 、 CpG Oligodeoxynucleotide 、 Quillaja 、 Scanning electron microscope 、 Chemistry 、 Zeta potential 、 Nuclear chemistry 、 Virology
摘要: Objective(s): The purpose of this study was preparation and evaluation PLGA nanospheres containing the influenza virus different adjuvants, Quillaja saponin (QS) CpG-ODN. Materials Methods: Nanospheres were prepared using double emulsion-solvent evaporation method. The morphological physicochemical properties studied by scanning electron microscopy (SEM), determination zeta potential, encapsulation efficiency release profile. Results: The particle size formulations less than 1000 nm, except for antigen. results confirmed with SEM images. Encapsulation antigen, QS CpG ODN 80%, 62% 31%, respectively. potential about -30 mV. burst observed all encapsulates reached to 48%, 44% 35% within 90 min CpG-ODN Qs content, Conclusion: The showed proper properties. These have good be used as delivery systems/adjuvants immunization against influenza.
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