Immunization against leishmaniasis by PLGA nanospheres loaded with an experimental autoclaved Leishmania major (ALM) and Quillaja saponins.
作者： M. Tafaghodi , A. Khamesipour , M. R. Jaafari , M. Kharazizadeh , M. Eskandari
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摘要: Immune responses against the Leishmania antigens are not sufficient to protect a leishmania challenge. Therefore these need be potentiated by various adjuvants and delivery systems. In this study, Poly (d,l-lactide-co-glycolide (PLGA) nanospheres as antigen system Quillaja saponins (QS) immunoadjuvant have been used enhance immune response autoclaved major (ALM). PLGA were prepared double-emulsion (W/O/W) technique. Particulate characteristics studied scanning electron microscopy particle size analysis. Mean diameter for loaded with ALM+QS was 294 ± 106 nm. BALB/c mice immunized three times in 3-weeks intervals using ALM plus QS [(ALM+QS)PLGA], encapsulated [(ALM)PLGA], (ALM)PLGA + QS, alone or PBS. The intensity of infection induced L. challenge assessed measuring footpad swelling. strongest protection, showed significantly (P < 0.05) smaller footpad, observed (ALM)PLGA. (ALM+QS)PLGA group least protection highest swelling, while (ALM)PLGA+QS, an intermediate no significant difference. IgG2a/IgG1 ratio 0.01), followed (ALM)PLGA+QS. IFN-γ lowest IL-4 production seen groups. parasite burden (ALM)PLGA+QS It is concluded that vaccine could increase protective responses, but adjuvant has reverse effect on presence adjuvant.
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