Cellular distribution of 3-hydroxy-3-methylglutaryl coenzyme A reductase and mevalonate kinase in leaves of Nepeta cataria
作者： Ronald E. Arebalo , Earl D. Mitchell
DOI: 10.1016/0031-9422(84)83068-X
关键词: Hydroxymethylglutaryl-CoA reductase 、 Mevalonate kinase 、 Enzyme 、 Coenzyme A 、 Differential centrifugation 、 Nepeta cataria 、 HMG-CoA reductase 、 Biology 、 Reductase 、 Biochemistry
摘要: Abstract In Nepeta cataria leaf tissue there are two separate activities of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and mevalonate (MVA) kinase respectively as determined by the use a 20–45% discontinuous sucrose density gradient. Cell-free extracts callus were prepared HMG-CoA MVA compared to in from porcine livers yeast autolysates. Callus N. has only one peak activity located at top Isolated chloroplast leaves have activity, near bottom autolysate also showed profile, Partial purification extract through differential centrifugation, 30–70% ammonium sulfate precipitation Bio-Gel P-100 column chromatography shows that kinase, 5-phosphomevalonate (MVAP) 5-pyrophosphomevalonate (MVAPP) decarboxylase remain same fractions. The extra-chloroplastidic may be separated centrifugation. These results suggest presence enzymes tissue—one second being extra-chloroplastidic.
elsevier.com
sci-hub.se 参考文章(19)
J. C. Gray, R. G. O. Kekwick, Mevalonate kinase in green leaves and etiolated cotyledons of the french bean Phaseolus vulgaris. Biochemical Journal. ,vol. 133, pp. 335- 347 ,(1973) , 10.1042/BJ1330335
LJ Rogers, SPJ Shah, TW Goodwin, Intracellular localization of mevalonate-activating enzymes in plant cells. Biochemical Journal. ,vol. 99, pp. 381- 388 ,(1966) , 10.1042/BJ0990381
Hirohiko KATSUKI, Isamu SHIMIZU, Jun NAGAI, Hiroshi HATANAKA, Eiki SAITO, Subcellular localization of 3-hydroxy-3-methylglutaryl-CoA reductase in Saccharomyces cerevisiae. Journal of Biochemistry. ,vol. 70, pp. 175- 177 ,(1971) , 10.1093/OXFORDJOURNALS.JBCHEM.A129616
Alan S. Beedle, Kenneth A. Munday, David C. Wilton, Studies on the biosynthesis of tetrahymanol in Tetrahymena pyriformis. The mechanism of inhibition by cholesterol Biochemical Journal. ,vol. 142, pp. 57- 64 ,(1974) , 10.1042/BJ1420057
Hiroshi Suzuki, Ikuzo Uritani, Subcellular localization of 3-hydroxy-3-methylglutaryl coenzyme A reductase and other membrane-bound enzymes in sweet potato roots1 Plant and Cell Physiology. ,vol. 17, pp. 691- 700 ,(1976) , 10.1093/OXFORDJOURNALS.PCP.A075325
S. Shefer, S. Hauser, V. Lapar, E.H. Mosbach, HMG CoA reductase of intestinal mucosa and liver of the rat Journal of Lipid Research. ,vol. 13, pp. 402- 412 ,(1972) , 10.1016/S0022-2275(20)39404-9
R. G. Jensen, J. A. Bassham, Photosynthesis by isolated chloroplasts Proceedings of the National Academy of Sciences of the United States of America. ,vol. 56, pp. 1095- 1101 ,(1966) , 10.1073/PNAS.56.4.1095
E.F. Hartree, Determination of protein: A modification of the lowry method that gives a linear photometric response Analytical Biochemistry. ,vol. 48, pp. 422- 427 ,(1972) , 10.1016/0003-2697(72)90094-2
Michael R. Downing, Earl D. Mitchell, Metabolism of mevalonic acid to phosphorylated intermediates in a cell-free extract from Nepeta cataria leaves Phytochemistry. ,vol. 13, pp. 1419- 1421 ,(1974) , 10.1016/0031-9422(74)80302-X
Howard M. Hill, Lyndon J. Rogers, Mevalonate activating enzymes and phosphatases in higher plants Phytochemistry. ,vol. 13, pp. 763- 777 ,(1974) , 10.1016/S0031-9422(00)91139-7