Soluble gp130 muteins with improved binding activity
作者： Stephanie Dr. Tenhumberg , Stefan Rose-John , Georg H. Wätzig , Jürgen Scheller , Joachim Grötzinger
DOI:
关键词: Chemistry 、 Soluble gp130 、 Amino acid residue 、 Stereochemistry 、 Monomer 、 Ligand (biochemistry) 、 Biological activity 、 Receptor 、 Glycoprotein 130
摘要: Described are soluble gp130 polypeptide monomers and dimers, wherein, in a preferred embodiment, at least one of the three amino acid residues Thr102 GIn113 or ASn114 N-terminal Ig-like domain is mutated to Tyr102, Phe113 Leu114, respectively. These mutations, alone combination, specifically enhance binding its ligand complex interleukin-6 receptor, thus increasing biological activity muteins. In particularly all mutations combined triple mutein Thr102Tyr/Gln113Phe/Asn114Leu (T102Y/Q113F/N114L). Moreover, pharmaceutical composition containing said dimers various medical uses described.
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