Improvement of L-lactic acid production by osmotic-tolerant mutant of Lactobacillus casei at high temperature.

摘要: L-Lactic acid production by Lactobacillus casei was used as a model to study the mechanism of substrate inhibition and strategy for enhancing L-lactic production. It found that concentration cell growth L-lactate decreased with increase glucose fermentation temperature. To enhance osmotic stress resistance strain at high temperature, mutant G-03 screened selected 360 g/L 45°C selective criterion. further lactic production, 3 biotin supplemented medium. As result, L: -lactate reached 198.2 (productivity 5.5 g L(-1) h(-1)) 41°C in 7-L fermentor 210 carbon source. -Lactate productivity were 115.2% 97.8% higher than those parent strain, respectively. The -lactic increasing temperature may provide an alternative approach organic other strains.