Properties of cloned human glioblastoma cells

摘要: Abstract We cloned a previously characterized glioblastoma-derived parent cell line (12–18) in order to obtain relatively homogenous population of human neural cells neoplastic origin. These reach high densities culture (over 100,000 cells/cm 2 ) and have mean DNA content per 18.1 ± 0.9 pg. A histogram the cells' chromosome numbers revealed one peak modal near diploid number 52, whereas had expressed polyploidy, with several peaks (including 52) at doubling level 16. Several consistent results were obtained by Giemsa staining. persistent structural alteration was duplication long arm #9 on another #9, translocation short #21. further observed that these secrete specific protease, plasminogen activator (PA), into serum-free medium (SFM). This enzyme assayed conversion purified plasmin subsequent degradation 125 I-labelled fibrin. Gioblastoma-derived higher levels cell-associated PA activity (2.9-fold) released more SFM (22-fold) than fetal cells. The presence this protease suggests mechanism for invasive character neoplasms (glioblastoma multiforme) vivo.