An improved bacterial test system for the detection and classification of mutagens and carcinogens

摘要: We previously described a set of four strains Salmonella typhimurium designed for detecting the various types mutagens, and showed their utility in wide variety carcinogens as mutagens. The lipopolysaccharide that normally coats these bacteria is barrier to penetration mutagens cell membrane. tester has been improved by adding mutation (rfa: deep rough) results deficient lipopolysaccharide. techniques using are presented tests shown be extremely sensitive convenient. specificity frameshift mutagenesis clarified. As adjuncts test with strains, we describe compares mutagenic killing rough without DNA excision repair, forward strain lacking repair.