Inhibition of Oral Carcinogenesis by a Protease Inhibitor

摘要: The effect of the Bowman-Birk inhibitor (BBI) and soybean trypsin (SBTI) on experimental 7,12-dimethylbenz[a]anthracene [(DMBA) CAS: 57-97-6]-induced oral carcinogenesis in Syrian male hamsters was examined. All treatments were applied topically both cheek pouches for 20 weeks, animals then sacrificed. Gross microscopic evaluations revealed a statistically significant reduction number invasive carcinomas, total tumors, tumor mass DMBA + BBI treatment group compared to treated with alone, autoclaved (a preparation which protease activity is destroyed), or SBTI. A (with use Boc-Val-Pro-Arg-MCA as substrate) measured found be elevated about tenfold tumorous nontumorous tissue from DMBA-treated pouches. This decreased but not SBTI groups, group. Partial characterization hydrolyzing diisopropyl fluorophosphate suggests that proteolytic serine protease. Iodoacetamide diethyl pyrocarbonate also inhibit enzyme activity, suggesting other residues may necessary catalysis, possibly including cysteine histidine. Our results suggest this play role DMBA-induced pouch carcinogenesis.