Direct evidence for cytotoxicity associated with expression of hepatitis delta virus antigen
作者： Susan M. Cole , Eric J. Gowans , Thomas B. Macnaughton , Pauline De La M. Hall , Christopher J. Burrell
关键词: Hepatitis 、 Virus antigen 、 Hepatitis D virus 、 Hepatitis D 、 Antigen Gene 、 Antigen 、 Hepatitis delta Antigens 、 Biology 、 Hepatitis B virus 、 Virology
摘要: It has been postulated that hepatocyte injury resulting from infection with hepatitis D virus may be caused by a direct cytotoxicity in contrast to immune-mediated associated B virus. We have transfected HeLa and HepG2 continuous cell lines recombinant plasmid containing the antigen gene under inducible control of human metallothionein promoter. The addition zinc culture medium then led expression with, short term, significant reduction rate RNA but not DNA synthesis and, longer death. necrotic cells had pyknotic nuclei shrunken eosinophilic cytoplasm; these resembled apoptotic bodies seen virus-related hepatitis. level individual produced changes was similar hepatocytes chimpanzee acute infection. conclude resulted cytotoxic cells, providing strong support for view specifically infected vivo.
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