Release characteristics and bioactivity of gelatin-tricalcium phosphate membranes covalently immobilized with nerve growth factors.

摘要: Abstract The gelatin-tricalcium phosphate membranes were cross-linking with low concentration glutaraldehyde solution (GTG). This material has good mechanical property, biocompatibility, and is feasible for surgical manipulation. For axonal regeneration, nerve growth factors (NGF) immobilized onto the composite (GTG) carbodiimide. purpose of this study was to evaluate release characteristics bioactivity NGF after covalent immobilization GTG (GEN). released from quantified using ELISA method. PC 12 cells cultured on GEN composites. Cell survival, cytotoxicity, cellular activity evaluated by total protein content, LDH activity, MTT assay respectively. Neurite outgrowth used biological composite. From measurement, releasing curve showing two distinctive parts different slopes indicated that in diffusion-controlled mechanism degradation-controlled While culturing cells, leakage results implied whether cross-linked or not showed little cytotoxicity. content lower than control group. However, 56%±3.98 significant neurite which statistically higher without immobilization. In addition, sustained bioactive months had been demonstrated assay. these experiments, it can be concluded technique present capable immobilizing covalently remaining NGF. Therefore, materials a candidate future therapeutic application repair.