Mechanobiology of engineered cartilage cultured under a quantified fluid-dynamic environment

摘要: Natural cartilage remodels both in vivo and vitro response to mechanical forces hence stimulation is believed have a potential as tool modulate extra-cellular matrix synthesis tissue-engineered cartilage. Fluid-induced shear known enhance chondrogenesis on animal cells. A well-defined hydrodynamic environment required study the biochemical of three-dimensional engineered cell systems. We developed perfused-column bioreactor which culture medium flows through chondrocyte-seeded porous scaffolds, together with computational fluid-dynamic model flow constructs' microstructure. preliminary experiment human chondrocyte growth under static versus dynamic conditions described. The median stress imposed cells culture, predicted by CFD model, 3 × 10−3 Pa (0.03 dyn/cm2) at rate 0.5 ml/min corresponding an inlet fluid velocity 44.2 μm/s. Providing yielded significant differences morphology construct structure.